Identification of Hop stunt viroid infecting Citrus limon in China using small RNAs deep sequencing approach.

ABSTRACT:

Background

The advent of next generation sequencing technology has allowed for significant advances in plant virus discovery, particularly for identification of covert viruses and previously undescribed viruses. The Citrus limon Burm. f. (C. limon) is a small evergreen tree native to Asia, and . China is the world's top lemon-producing nation.

Findings

In this work, lemon samples were collected from southwestern of China, where an unknown disease outbreak had caused huge losses in the lemon production industry. Using high-throughput pyrosequencing and the assembly of small RNAs, we showed that the Hop stunt viroid (HSVd) was present in C. limon leaf sample. The majority of it is a main lemon producing agricultural cultivarHop stunt viroid derived siRNAs (HSVd-siRNAs) in C. l

SUBMITTER: Su X 

PROVIDER: S-EPMC4492010 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature