Evaluating the effects of preanalytical variables on the stability of the human plasma proteome.

ABSTRACT: High quality clinical biospecimens are vital for biomarker discovery, verification, and validation. Variations in blood processing and handling can affect protein abundances and assay reliability. Using an untargeted LC-MS approach, we systematically measured the impact of preanalytical variables on the plasma proteome. Time prior to processing was the only variable that affected the plasma protein levels. LC-MS quantification showed that preprocessing times <6h had minimal effects on the immunodepleted plasma proteome, but by 4 days significant changes were apparent. Elevated levels of many proteins were observed, suggesting that in addition to proteolytic degradation during the preanalytical phase, changes in protein structure are also important considerations for protocols using antibod

SUBMITTER: Hassis ME 

PROVIDER: S-EPMC4492164 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature