Proteomic profiling of human sera for discovery of potential biomarkers to monitor abstinence from alcohol abuse.

ABSTRACT: Although numerous biomarkers or biomarker candidates have been discovered to detect levels of drinking and intervals of time after last drinking episode, only a few biomarkers have been applied to monitor abstinence in a longer interval (≥6 wks) from alcohol abuse. Considering sample sources, sensitivity, and specificity, new biomarkers from blood with better accuracy are needed. To address this, serum proteomic profiles were compared between pre- and post- treatment samples from subjects seeking treatment for alcohol abuse and dependence in an intensive 6 wk daily outpatient program using high-abundance plasma protein immunodepletion and LC-MS/MS techniques. Protein identification, quantification, candidate biomarker selection, and prioritization analyses were carried out. Among the 246 q

SUBMITTER: Lai X 

PROVIDER: S-EPMC4492280 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature