Constitutive and Inducible Expression of Invasion-related Factors in PC-3 Prostate Cancer Cells.

ABSTRACT:

Background

Tumor growth and invasion are interconnected with the tumor microenvironment. Overexpression of genes that regulate cancer cell invasion by growth factors, cytokines, and lipid factors can affect cancer aggressiveness. A comparative gene expression analysis between highly invasive and low invasive cells revealed that various genes are differentially expressed in association with invasive potential. In this study, we selected variant PC-3 prostate cancer cell sublines and discovered critical molecules that contributed to their invasive potential.

Methods

The high invasive and low invasive variant PC-3 cell sublines were obtained by serial selection following Matrigel-coated Transwell invasion and were characterized by Transwell invasion, luciferase reporter assay,

SUBMITTER: Hwang YS 

PROVIDER: S-EPMC4492356 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature