Conserved Noncoding Sequences Regulate lhx5 Expression in the Zebrafish Forebrain.

ABSTRACT: The LIM homeobox family protein Lhx5 plays important roles in forebrain development in the vertebrates. The lhx5 gene exhibits complex temporal and spatial expression patterns during early development but its transcriptional regulation mechanisms are not well understood. Here, we have used transgenesis in zebrafish in order to define regulatory elements that drive lhx5 expression in the forebrain. Through comparative genomic analysis we identified 10 non-coding sequences conserved in five teleost species. We next examined the enhancer activities of these conserved non-coding sequences with Tol2 transposon mediated transgenesis. We found a proximately located enhancer gave rise to robust reporter EGFP expression in the forebrain regions. In addition, we identified an enhancer located at app

SUBMITTER: Sun L 

PROVIDER: S-EPMC4492605 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature