Flow cytometry for intracellular SPION quantification: specificity and sensitivity in comparison with spectroscopic methods.

ABSTRACT: Due to their special physicochemical properties, iron nanoparticles offer new promising possibilities for biomedical applications. For bench to bedside translation of super-paramagnetic iron oxide nanoparticles (SPIONs), safety issues have to be comprehensively clarified. To understand concentration-dependent nanoparticle-mediated toxicity, the exact quantification of intracellular SPIONs by reliable methods is of great importance. In the present study, we compared three different SPION quantification methods (ultraviolet spectrophotometry, magnetic particle spectroscopy, atomic adsorption spectroscopy) and discussed the shortcomings and advantages of each method. Moreover, we used those results to evaluate the possibility to use flow cytometric technique to determine the cellular SPION co

SUBMITTER: Friedrich RP 

PROVIDER: S-EPMC4492632 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature