Ontology highlight
ABSTRACT:
SUBMITTER: Cloutier P
PROVIDER: S-EPMC4492713 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Methods (San Diego, Calif.) 20090518 4
Thirty years of research on gene transcription has uncovered a myriad of factors that regulate, directly or indirectly, the activity of RNA polymerase II (RNAPII) during mRNA synthesis. Yet many regulatory factors remain to be discovered. Using protein affinity purification coupled to mass spectrometry (AP-MS), we recently unraveled a high-density interaction network formed by RNAPII and its accessory factors from the soluble fraction of human cell extracts. Validation of the dataset using a mac ...[more]