Reversible Dissolution of Microdomains in Detergent-Resistant Membranes at Physiological Temperature.

ABSTRACT: The formation of lipid microdomains ("rafts") is presumed to play an important role in various cellular functions, but their nature remains controversial. Here we report on microdomain formation in isolated, detergent-resistant membranes from MDA-MB-231 human breast cancer cells, studied by atomic force microscopy (AFM). Whereas microdomains were readily observed at room temperature, they shrunk in size and mostly disappeared at higher temperatures. This shrinking in microdomain size was accompanied by a gradual reduction of the height difference between the microdomains and the surrounding membrane, consistent with the behaviour expected for lipids that are laterally segregated in liquid ordered and liquid disordered domains. Immunolabeling experiments demonstrated that the microdomains c

SUBMITTER: Cremona A 

PROVIDER: S-EPMC4493071 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature