A signal-on fluorosensor based on quench-release principle for sensitive detection of antibiotic rapamycin.

ABSTRACT: An antibiotic rapamycin is one of the most commonly used immunosuppressive drugs, and also implicated for its anti-cancer activity. Hence, the determination of its blood level after organ transplantation or tumor treatment is of great concern in medicine. Although there are several rapamycin detection methods, many of them have limited sensitivity, and/or need complicated procedures and long assay time. As a novel fluorescent biosensor for rapamycin, here we propose "Q'-body", which works on the fluorescence quench-release principle inspired by the antibody-based quenchbody (Q-body) technology. We constructed rapamycin Q'-bodies by linking the two interacting domains FKBP12 and FRB, whose association is triggered by rapamycin. The fusion proteins were each incorporated position-specificall

SUBMITTER: Jeong HJ 

PROVIDER: S-EPMC4493541 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature