Using quantitative PCR with retrotransposon-based insertion polymorphisms as markers in sugarcane.

ABSTRACT: Sugarcane is the main source of the world's sugar and is becoming increasingly important as a source of biofuel. The highly polyploid and heterozygous nature of the sugarcane genome has meant that characterization of the genome has lagged behind that of other important crops. Here we developed a method using a combination of quantitative PCR with a transposable marker system to score the relative number of alleles with a transposable element (TE) present at a particular locus. We screened two genera closely related to Saccharum (Miscanthus and Erianthus), wild Saccharum, traditional cultivars, and 127 modern cultivars from Brazilian and Australian breeding programmes. We showed how this method could be used in various ways. First, we showed that the method could be extended to be used as p

SUBMITTER: Metcalfe CJ 

PROVIDER: S-EPMC4493790 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature