Lipopolysaccharide challenge significantly influences lipid metabolism and proteome of white adipose tissue in growing pigs.

ABSTRACT:

Background

White adipose tissue is recognized as a highly active organ, which is closely related to a large number of physiological and metabolic processes besides storing triglycerides. However, little is known regarding the response of adipose tissue to acute inflammation. Therefore, in this study we employed growing pigs to investigate the changes of lipid metabolism and proteome in white adipose tissue after lipopolysaccharide (LPS) stimulation as a model for bacterial infection.

Methods

The expression of lipid metabolism and inflammation related genes was determined by quantitative real-time polymerase chain reaction. Label-free proteomics analysis was used to investigate changes of the protein profile in white adipose tissue and western blot was used to verify changes

SUBMITTER: Guo J 

PROVIDER: S-EPMC4493945 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature