Integration of microRNA signatures of distinct mammary epithelial cell types with their gene expression and epigenetic portraits.

ABSTRACT:

Introduction

MicroRNAs (miRNAs) have been implicated in governing lineage specification and differentiation in multiple organs; however, little is known about their specific roles in mammopoiesis. We have determined the global miRNA expression profiles of functionally distinct epithelial subpopulations in mouse and human mammary tissue, and compared these to their cognate transcriptomes and epigenomes. Finally, the human miRNA signatures were used to interrogate the different subtypes of breast cancer, with a view to determining miRNA networks deregulated during oncogenesis.

Methods

RNA from sorted mouse and human mammary cell subpopulations was subjected to miRNA expression analysis using the TaqMan MicroRNA Array. Differentially expressed (DE) miRNAs were correlated with g

SUBMITTER: Pal B 

PROVIDER: S-EPMC4497411 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature