Enhanced Autophagy of Adipose-Derived Stem Cells Grown on Chitosan Substrates.

ABSTRACT: Autophagy is an important protein quality control mechanism for cells under stress conditions to promote cell survival. Modulation of autophagy on biomaterial substrates is rarely reported. In this study, the autophagy of adipose-derived stem cells (ADSCs) cultured on chitosan (CS) substrates was examined. Compared to the traditional monolayer culture, ADSCs cultured on CS substrates showed spheroid formation as well as a prolonged upregulation of autophagosomal marker-microtubule-associated protein 1 light chain 3 (LC3) II protein expression. In addition, the green fluorescent protein tagged-LC3 (GFP-LC3) expressing ADSCs also revealed more GFP-LC3 puncta on CS substrates. The enhanced autophagy on CS substrates was associated with Ca(2+), while ethylene glycol tetraacetic acid (EGTA), a

SUBMITTER: Yang CM 

PROVIDER: S-EPMC4497627 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature