Conformational thermostabilisation of corticotropin releasing factor receptor 1.

ABSTRACT: Recent technical advances have greatly facilitated G-protein coupled receptors crystallography as evidenced by the number of successful x-ray structures that have been reported recently. These technical advances include novel detergents, specialised crystallography techniques as well as protein engineering solutions such as fusions and conformational thermostabilisation. Using conformational thermostabilisation, it is possible to generate variants of GPCRs that exhibit significantly increased stability in detergent micelles whilst preferentially occupying a single conformation. In this paper we describe for the first time the application of this technique to a member of a class B GPCR, the corticotropin releasing factor receptor 1 (CRF1R). Mutational screening in the presence of the invers

SUBMITTER: Kean J 

PROVIDER: S-EPMC4498186 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature