Development of Multiplex PCR for Simultaneous Detection of Three Pathogenic Shigella Species.

ABSTRACT:

Background

Shigella species are among the common causes of bacterial diarrhoeal diseases. Traditional detection methods are time-consuming resulting in delay in treatment and control of Shigella infections thus there is a need to develop molecular methods for rapid and simultaneous detection of Shigella spp. In this study a rapid multiplex PCR were developed for simultaneous detection of three pathogenic Shigella species.

Methods

For detection of Shigella spp., a pair of primers was used to replicate a chromosomal sequence. Three other sets of primers were also designed to amplify the target genes of three most common species of Shigella in Iran including S. sonnei, S. flexneri and S. boydii. The multiplex PCR assay was optimized for simultaneous detection and differentiatio

SUBMITTER: Ranjbar R 

PROVIDER: S-EPMC4499087 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature