De novo assembly of a transcriptome from juvenile blue crabs (Callinectes sapidus) following exposure to surrogate Macondo crude oil.

ABSTRACT:

Background

The blue crab, Callinectes sapidus, is economically and ecologically important in western Atlantic and Gulf of Mexico coastal estuaries. In 2010 blue crabs in the northern Gulf of Mexico were exposed to crude oil and chemical dispersants from the Deepwater Horizon oil spill. To characterize the blue crab transcriptome and identify genes that could be regulated in response to oil exposure we sequenced transcriptomes from hepatopancreas and gill tissues of juvenile blue crabs after exposing them to a water-accommodated fraction of surrogate Macondo crude oil in the laboratory and compared them to transcriptomes from an unexposed control group.

Results

Illumina sequencing provided 42.5 million paired-end sequencing reads for the control group and 44.9 million paired-

SUBMITTER: Yednock BK 

PROVIDER: S-EPMC4499174 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature