Changes in gene expression in liver tissue from patients with fulminant hepatitis E.

ABSTRACT:

Aim

To study host gene expression and number of immune cells in liver tissues from patients with fulminant hepatitis E (FH-E).

Methods

Microarray-based expression profiling was done using Illumina Human WG-6_v3_BeadChip arrays on post-mortem liver tissue from 5 patients with FH-E, and compared with similar tissue from 6 patients with fulminant hepatitis B (FH-B; disease controls) and normal liver tissue from 6 persons. Differential expression was defined as ≥ 2.0-fold change with Benjamini-Hochberg false discovery rate below 0.05 using t-test in liver tissue from FH-B and FH-E, than healthy liver tissue. For some genes that showed differential expression in FH-E, microarray data were validated using quantitative reverse transcription PCR. Differentially expressed gene lists

SUBMITTER: Naik A 

PROVIDER: S-EPMC4499345 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature