Label-free profiling of white adipose tissue of rats exhibiting high or low levels of intrinsic exercise capacity.

ABSTRACT: Divergent selection has created rat phenotypes of high- and low-capacity runners (HCR and LCR, respectively) that have differences in aerobic capacity and correlated traits such as adiposity. We analyzed visceral adipose tissue of HCR and LCR using label-free high-definition MS (elevated energy) profiling. The running capacity of HCR was ninefold greater than LCR. Proteome profiling encompassed 448 proteins and detected 30 significant (p <0.05; false discovery rate <10%, calculated using q-values) differences. Approximately half of the proteins analyzed were of mitochondrial origin, but there were no significant differences in the abundance of proteins involved in aerobic metabolism. Instead, adipose tissue of LCR rats exhibited greater abundances of proteins associated with adipogenesis (

SUBMITTER: Bowden-Davies K 

PROVIDER: S-EPMC4500166 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature