Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure.

ABSTRACT:

Objectives

Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance.

Methods

Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance.

Results

All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenot

SUBMITTER: Webber MA 

PROVIDER: S-EPMC4500774 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature