Urinary 1H-NMR metabolomics can distinguish pancreatitis patients from healthy controls.

ABSTRACT:

Context

The characterization of the urinary metabolome may yield biomarkers indicative of pancreatitis.

Objectives

We establish a non-invasive technique to compare urinary metabolic profiles in patients with acute and chronic pancreatitis to healthy controls.

Methods

Urine was obtained from healthy controls (HC, n=5), inpatients with mild acute pancreatitis (AP, n=5), and outpatients with chronic pancreatitis (CP, n=5). Proton nuclear magnetic resonance spectra were obtained for each sample. Metabolites were identified and quantified in each spectrum; resulting concentrations were normalized to account for differences in dilution among samples. Kruskal-Wallis test, post-hoc Mann-Whitney U tests, and principal component analysis were performed to identify metabolites t

SUBMITTER: Lusczek ER 

PROVIDER: S-EPMC4500934 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature