Analysis of Deregulated microRNAs and Their Target Genes in Gastric Cancer.

ABSTRACT:

Background

MicroRNAs (miRNAs) are widely studied non-coding RNAs that modulate gene expression. MiRNAs are deregulated in different tumors including gastric cancer (GC) and have potential diagnostic and prognostic implications. The aim of our study was to determine miRNA profile in GC tissues, followed by evaluation of deregulated miRNAs in plasma of GC patients. Using available databases and bioinformatics methods we also aimed to evaluate potential target genes of confirmed differentially expressed miRNA and validate these findings in GC tissues.

Methods

The study included 51 GC patients and 51 controls. Initially, we screened miRNA expression profile in 13 tissue samples of GC and 12 normal gastric tissues with TaqMan low density array (TLDA). In the second stage, differe

SUBMITTER: Juzenas S 

PROVIDER: S-EPMC4501563 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature