Systems-wide analysis of BCR signalosomes and downstream phosphorylation and ubiquitylation.

ABSTRACT: B-cell receptor (BCR) signaling is essential for the development and function of B cells; however, the spectrum of proteins involved in BCR signaling is not fully known. Here we used quantitative mass spectrometry-based proteomics to monitor the dynamics of BCR signaling complexes (signalosomes) and to investigate the dynamics of downstream phosphorylation and ubiquitylation signaling. We identify most of the previously known components of BCR signaling, as well as many proteins that have not yet been implicated in this system. BCR activation leads to rapid tyrosine phosphorylation and ubiquitylation of the receptor-proximal signaling components, many of which are co-regulated by both the modifications. We illustrate the power of multilayered proteomic analyses for discovering novel BCR si

SUBMITTER: Satpathy S 

PROVIDER: S-EPMC4501846 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature