Astrocytes in the optic nerve head express putative mechanosensitive channels.

ABSTRACT:

Purpose

To establish whether optic nerve head astrocytes express candidate molecules to sense tissue stretch.

Methods

We used conventional PCR, quantitative PCR, and single-cell reverse transcription PCR (RT-PCR) to assess the expression of various members of the transient receptor potential (TRP) channel family and of the recently characterized mechanosensitive channels Piezo1 and 2 in optic nerve head tissue and in single, isolated astrocytes.

Results

Most TRP subfamilies (TRPC, TRPM, TRPV, TRPA, and TRPP) and Piezo1 and 2 were expressed in the optic nerve head of the mouse. Quantitative real-time PCR analysis showed that TRPC1, TRPM7, TRPV2, TRPP2, and Piezo1 are the dominant isoforms in each subfamily. Single-cell RT-PCR revealed that many TRP isoforms, TRPC1-2, T

SUBMITTER: Choi HJ 

PROVIDER: S-EPMC4502055 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature