Human scFv antibody fragments specific for hepatocellular carcinoma selected from a phage display library.

ABSTRACT:

Aim

To identify the scFv antibody fragments specific for hepatocellular carcinoma by biopanning from a large human naive scFv phage display library.

Methods

A large human naive scFv phage library was used to search for the specific targets by biopanning with the hepatocellular carcinoma cell line HepG2 for the positive-selecting and the normal liver cell line L02 for the counter-selecting. After three rounds of biopanning, individual scFv phages binding selectively to HepG2 cells were picked out. PCR was carried out for identification of the clones containing scFv gene sequence. The specific scFv phages were selected by ELISA and flow cytometry. DNA sequences of positive clones were analyzed by using Applied Biosystem Automated DNA sequencers 3 730. The expression proteins o

SUBMITTER: Yu B 

PROVIDER: S-EPMC4502091 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature