Improved separation and detection of picolinic acid and quinolinic acid by capillary electrophoresis-mass spectrometry: application to analysis of human cerebrospinal fluid.

ABSTRACT: "Quinolinic acid (QA)", a metabolite of the kynurenine pathway (KP), is implicated as a major neurological biomarker, which causes inflammatory disorders, whereas there is an increase evidence of the role of picolinic acid (PA) in neuroinflammation. Therefore, there is an urgent need to develop new clinical test for early diagnosis of neuroinflammatory disorders. A comparison is made between three different platforms such as high performance liquid chromatography-electrospray mass spectrometry (HPLC-ESI-MS/MS), nano LC-Chip/ESI-MS/MS, as well as the use of cationic (quaternary ammonium) and anionic (sulfonated) coated capillaries in capillary electrophoresis (CE)-ESI-MS/MS. The comparison revealed that CE-ESI-MS/MS method using a quaternary ammonium coated capillary is the best method for

SUBMITTER: Wang X 

PROVIDER: S-EPMC4502419 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature