Libraries for two-hybrid screening of yeast and hyphal growth forms in Zymoseptoria tritici.

ABSTRACT: Pathogenic fungi are constantly emerging resistance to anti-fungal treatments. Therefore, identification of new fungicide targets is important. Good candidates are essential fungal proteins and their regulators. An efficient way to reveal the molecular environment of an essential protein is the search for interacting factors. Here, we establish three yeast two-hybrid libraries, covering yeast and hyphal stages of the wheat pathogen Zymoseptoria tritici. No detectable genomic DNA was present in any of the 3 libraries. Random amplification revealed that the libraries include cDNA fragments of up to 2000bp, suggesting that small-to-medium sized proteins are represented therein. Indeed, full-length cDNAs of five proteins were found in all libraries. The full-length cDNA of large chitin synthas

SUBMITTER: Ma W 

PROVIDER: S-EPMC4502458 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature