Isolation, production and characterization of fully human monoclonal antibodies directed to Plasmodium falciparum MSP10.

ABSTRACT:

Background

Semi-immunity against the malaria parasite is defined by a protection against clinical episodes of malaria and is partially mediated by a repertoire of inhibitory antibodies directed against the blood stage of Plasmodium falciparum, in particular against surface proteins of merozoites, the invasive form of the parasite. Such antibodies may be used for preventive or therapeutic treatment of P. falciparum malaria. Here, the isolation and characterization of novel human monoclonal antibodies (humAbs) for such applications is described.

Methods

B lymphocytes had been selected by flow cytometry for specificity against merozoite surface proteins, including the merozoite surface protein 10 (MSP10). After Epstein-Barr virus (EBV) transformation and identification of promi

SUBMITTER: Maskus DJ 

PROVIDER: S-EPMC4502606 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature