Production, secretion and purification of a correctly folded staphylococcal antigen in Lactococcus lactis.

ABSTRACT:

Background

Lactococcus lactis, a lactic acid bacterium traditionally used to ferment milk and manufacture cheeses, is also, in the biotechnology field, an interesting host to produce proteins of medical interest, as it is "Generally Recognized As Safe". Furthermore, as L. lactis naturally secretes only one major endogenous protein (Usp45), the secretion of heterologous proteins in this species facilitates their purification from a protein-poor culture medium. Here, we developed and optimized protein production and secretion in L. lactis to obtain proteins of high quality, both correctly folded and pure to a high extent. As proteins to be produced, we chose the two transmembrane members of the HtrA protease family in Staphylococcus aureus, an important extra-cellular pathogen, as th

SUBMITTER: Samazan F 

PROVIDER: S-EPMC4502909 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature