Transcription Profiles of Marker Genes Predict The Transdifferentiation Relationship between Eight Types of Liver Cell during Rat Liver Regeneration.

ABSTRACT:

Objective

To investigate the transdifferentiation relationship between eight types of liver cell during rat liver regeneration (LR).

Materials and methods

114 healthy Sprague-Dawley (SD) rats were used in this experimental study. Eight types of liver cell were isolated and purified with percoll density gradient centrifugation and immunomagentic bead methods. Marker genes for eight types of cell were obtained by retrieving the relevant references and databases. Expression changes of markers for each cell of the eight cell types were measured using microarray. The relationships between the expression profiles of marker genes and transdifferentiation among liver cells were analyzed using bioinformatics. Liver cell transdifferentiation was predicted by comparing expression profi

SUBMITTER: Chen X 

PROVIDER: S-EPMC4503848 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature