High resolution identity testing of inactivated poliovirus vaccines.

ABSTRACT:

Background

Definitive identification of poliovirus strains in vaccines is essential for quality control, particularly where multiple wild-type and Sabin strains are produced in the same facility. Sequence-based identification provides the ultimate in identity testing and would offer several advantages over serological methods.

Methods

We employed random RT-PCR and high throughput sequencing to recover full-length genome sequences from monovalent and trivalent poliovirus vaccine products at various stages of the manufacturing process.

Results

All expected strains were detected in previously characterised products and the method permitted identification of strains comprising as little as 0.1% of sequence reads. Highly similar Mahoney and Sabin 1 strains were readily dis

SUBMITTER: Mee ET 

PROVIDER: S-EPMC4504004 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature