Suppressing mutation-induced protein aggregation in mammalian cells by mutating residues significantly displaced upon the original mutation.

ABSTRACT: Mutations introduced to wild-type proteins naturally, or intentionally via protein engineering, often lead to protein aggregation. In particular, protein aggregation within mammalian cells has significant implications in the disease pathology and biologics production; making protein aggregation modulation within mammalian cells a very important engineering topic. Previously, we showed that the semi-rational design approach can be used to reduce the intracellular aggregation of a protein by recovering the conformational stability that was lowered by the mutation. However, this approach has limited utility when no rational design approach to enhance conformational stability is readily available. In order to overcome this limitation, we investigated whether the modification of residues signif

SUBMITTER: Gregoire S 

PROVIDER: S-EPMC4504021 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature