Defined Nutrient Diets Alter Susceptibility to Clostridium difficile Associated Disease in a Murine Model.

ABSTRACT:

Background

Clostridium difficile is a major identifiable and treatable cause of antibiotic-associated diarrhea. Poor nutritional status contributes to mortality through weakened host defenses against various pathogens. The primary goal of this study was to assess the contribution of a reduced protein diet to the outcomes of C. difficile infection in a murine model.

Methods

C57BL/6 mice were fed a traditional house chow or a defined diet with either 20% protein or 2% protein and infected with C. difficile strain VPI10463. Animals were monitored for disease severity, clostridial shedding and fecal toxin levels. Select intestinal microbiota were measured in stool and C. difficile growth and toxin production were quantified ex vivo in intestinal contents from untreated or antibi

SUBMITTER: Moore JH 

PROVIDER: S-EPMC4504475 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature