Global promoter methylation analysis reveals novel candidate tumor suppressor genes in natural killer cell lymphoma.

ABSTRACT:

Purpose

To identify tumor suppressor genes epigenetically silenced by promoter hypermethylation in extranodal natural killer cell lymphoma (NKCL).

Experimental design

Promoter methylation was analyzed with global and locus-specific methylation assays in NKCL cases and NK cell lines. Gene expression profiles were used to identify genes for which aberrant promoter methylation was associated with transcriptional silencing. Selected DNA methylations were validated by RRBS, pyrosequencing, or q-MSP. Decitabine treatment was performed to evaluate reactivation of methylated genes. The tumor suppressor effect of silenced genes was evaluated functionally by reintroducing them into NK cell lines.

Results

We observed significant promoter hypermethylation in most NKCL samples com

SUBMITTER: Kucuk C 

PROVIDER: S-EPMC4504988 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature