Folded or Not? Tracking Bet v 1 Conformation in Recombinant Allergen Preparations.

ABSTRACT:

Background

Recombinant Bet v 1a (rBet v 1a) has been used in allergy research for more than three decades, including clinical application of so-called hypoallergens. Quantitative IgE binding to rBet v 1a depends on its native protein conformation, which might be compromised upon heterologous expression, purification, or mutational engineering of rBet v 1a.

Objective

To correlate experimental/theoretical comparisons of IgE binding of defined molar ratios of folded/misfolded recombinant Bet v 1a variants and to determine accuracy and precision of immuno- and physicochemical assays routinely used to assess the quality of recombinant allergen preparations.

Methods

rBet v 1a and its misfolded variant rBet v 1aS112P/R145P were heterologously expressed and purified from Esch

SUBMITTER: Husslik F 

PROVIDER: S-EPMC4506129 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature