The von Hippel-Lindau tumour suppressor gene: uncovering the expression of the pVHL172 isoform.

ABSTRACT:

Background

The von Hippel-Lindau (VHL) gene encodes two mRNA variants. Variant 1 encodes two protein isoforms, pVHL213 and pVHL160, that have been extensively documented in the literature. Variant 2 is produced by alternative splicing of exon 2 and encodes a pVHL isoform of 172 amino acids with a theoretical molecular weight of 19 kDa (pVHL172), the expression of which has never been demonstrated so far due to the absence of suitable antibodies.

Methods

We have generated an anti-pVHL monoclonal antibody (JD-1956) using pVHL172 recombinant protein. We tested the antibody against exogenous or endogenous expressed proteins in different cell lines. We identified the pVHL172 using a silencing RNA strategy. The epitope of the antibody was mapped using a peptide array.

Results</

SUBMITTER: Chesnel F 

PROVIDER: S-EPMC4506380 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature