Exosomal microRNA in serum is a novel biomarker of recurrence in human colorectal cancer.

ABSTRACT:

Background

Functional microRNAs (miRNAs) in exosomes have been recognised as potential stable biomarkers in cancers. The aim of this study is to identify specific miRNAs in exosome as serum biomarkers for the early detection of recurrence in human colorectal cancer (CRC).

Methods

Serum samples were sequentially obtained from six patients with and without recurrent CRC. The miRNAs were purified from exosomes, and miRNA microarray analysis was performed. The miRNA expression profiles and copy number aberrations were explored using microarray and array CGH analyses in 124 CRC tissues. Then, we validated exosomal miRNAs in 2 serum sample sets (90 and 209 CRC patients) by quantitative real-time RT-PCR.

Results

Exosomal miR-17-92a cluster expression level in serum was corre

SUBMITTER: Matsumura T 

PROVIDER: S-EPMC4506387 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature