Decellularized skeletal muscle as an in vitro model for studying drug-extracellular matrix interactions.

ABSTRACT: Several factors can affect drug absorption after intramuscular (IM) injection: drug solubility, drug transport across cell membranes, and drug metabolism at the injection site. We found that potential interactions between the drug and the extracellular matrix (ECM) at the injection site can also affect the rate of absorption post-injection. Using decellularized skeletal muscle, we developed a simple method to model drug absorption after IM injection, and showed that the nature of the drug-ECM interaction could be investigated by adding compounds that alter binding. We validated the model using the vitamin B12 analog cobinamide with different bound ligands. Cobinamide is being developed as an IM injectable treatment for cyanide poisoning, and we found that the in vitro binding data correlat

SUBMITTER: Wassenaar JW 

PROVIDER: S-EPMC4506649 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature