Chromosomal Arrangement of Phosphorelay Genes Couples Sporulation and DNA Replication.

ABSTRACT: Genes encoding proteins in a common regulatory network are frequently located close to one another on the chromosome to facilitate co-regulation or couple gene expression to growth rate. Contrasting with these observations, here, we demonstrate a functional role for the arrangement of Bacillus subtilis sporulation network genes on opposite sides of the chromosome. We show that the arrangement of two sporulation network genes, one located close to the origin and the other close to the terminus, leads to a transient gene dosage imbalance during chromosome replication. This imbalance is detected by the sporulation network to produce cell-cycle coordinated pulses of the sporulation master regulator Spo0A∼P. This pulsed response allows cells to decide between sporulation and continued vegetativ

SUBMITTER: Narula J 

PROVIDER: S-EPMC4506695 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature