Analysis of endoscopic pancreatic function test (ePFT)-collected pancreatic fluid proteins precipitated via ultracentrifugation.

ABSTRACT:

Context

We have shown previously that trichloroacetic acid precipitation is an effective method of protein extraction from pancreatic fluid for downstream biomarker discovery, compared to other common extraction methods tested.

Objective

We aim to assess the utility of ultracentrifugation as an alternative method of protein extraction from pancreatic fluid.

Design

Proteins extracted from trichloroacetic acid- and ultracentrifugation-precipitated pancreatic fluid were identified using mass spectrometry techniques (in-gel tryptic digestion followed by liquid chromatography-tandem mass spectrometry; GeLC-MS/MS). Data were analyzed using Proteome Discoverer and Scaffold 3.

Setting

This is a proteomic analysis experiment of endoscopically collected fluid in an acade

SUBMITTER: Paulo JA 

PROVIDER: S-EPMC4506755 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature