Domain-Specific and Stage-Intrinsic Changes in Tcrb Conformation during Thymocyte Development.

ABSTRACT: Considerable cross-talk exists between mechanisms controlling genome architecture and gene expression. AgR loci are excellent models for these processes because they are regulated at both conformational and transcriptional levels to facilitate their assembly by V(D)J recombination. Upon commitment to the double-negative stage of T cell development, Tcrb adopts a compact conformation that promotes long-range recombination between Vβ gene segments (Trbvs) and their DβJβ targets. Formation of a functional VβDβJβ join signals for robust proliferation of double-negative thymocytes and their differentiation into double-positive (DP) cells, where Trbv recombination is squelched (allelic exclusion). DP differentiation also is accompanied by decontraction of Tcrb, which has been thought to separate

SUBMITTER: Majumder K 

PROVIDER: S-EPMC4506872 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature