MMP-13 In-Vivo Molecular Imaging Reveals Early Expression in Lung Adenocarcinoma.

ABSTRACT:

Introduction

Several matrix metalloproteinases (MMPs) are overexpressed in lung cancer and may serve as potential targets for the development of bioactivable probes for molecular imaging.

Objective

To characterize and monitor the activity of MMPs during the progression of lung adenocarcinoma.

Methods

K-rasLSL-G12D mice were imaged serially during the development of adenocarcinomas using fluorescence molecular tomography (FMT) and a probe specific for MMP-2, -3, -9 and -13. Lung tumors were identified using FMT and MRI co-registration, and the probe concentration in each tumor was assessed at each time-point. The expression of Mmp2, -3, -9, -13 was quantified by qRT-PCR using RNA isolated from microdissected tumor cells. Immunohistochemical staining of overexpressed MM

SUBMITTER: Salaun M 

PROVIDER: S-EPMC4508003 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature