Probing the Biology of Giardia intestinalis Mitosomes Using In Vivo Enzymatic Tagging.

ABSTRACT: Giardia intestinalis parasites contain mitosomes, one of the simplest mitochondrion-related organelles. Strategies to identify the functions of mitosomes have been limited mainly to homology detection, which is not suitable for identifying species-specific proteins and their functions. An in vivo enzymatic tagging technique based on the Escherichia coli biotin ligase (BirA) has been introduced to G. intestinalis; this method allows for the compartment-specific biotinylation of a protein of interest. Known proteins involved in the mitosomal protein import were in vivo tagged, cross-linked, and used to copurify complexes from the outer and inner mitosomal membranes in a single step. New proteins were then identified by mass spectrometry. This approach enabled the identification of highly div

SUBMITTER: Martincova E 

PROVIDER: S-EPMC4508323 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature