A genome-wide CRISPR library for high-throughput genetic screening in Drosophila cells.

ABSTRACT: The simplicity of the CRISPR/Cas9 system of genome engineering has opened up the possibility of performing genome-wide targeted mutagenesis in cell lines, enabling screening for cellular phenotypes resulting from genetic aberrations. Drosophila cells have proven to be highly effective in identifying genes involved in cellular processes through similar screens using partial knockdown by RNAi. This is in part due to the lower degree of redundancy between genes in this organism, whilst still maintaining highly conserved gene networks and orthologs of many human disease-causing genes. The ability of CRISPR to generate genetic loss of function mutations not only increases the magnitude of any effect over currently employed RNAi techniques, but allows analysis over longer periods of time which c

SUBMITTER: Bassett AR 

PROVIDER: S-EPMC4508376 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature