Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome Sequencing.

ABSTRACT: Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.

SUBMITTER: Bjorn-Mortensen K 

PROVIDER: S-EPMC4508433 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature