Dexamethasone Stiffens Trabecular Meshwork, Trabecular Meshwork Cells, and Matrix.

ABSTRACT:

Purpose

Treatment with corticosteroids can result in ocular hypertension and may lead to the development of steroid-induced glaucoma. The extent to which biomechanical changes in trabecular meshwork (TM) cells and extracellular matrix (ECM) contribute toward this dysfunction is poorly understood.

Methods

Primary human TM (HTM) cells were cultured for either 3 days or 4 weeks in the presence or absence of dexamethasone (DEX), and cell mechanics, matrix mechanics and proteomics were determined, respectively. Adult rabbits were treated topically with either 0.1% DEX or vehicle over 3 weeks, and mechanics of the TM were determined.

Results

Treatment with DEX for 3 days resulted in a 2-fold increase in HTM cell stiffness, and this correlated with activation of extracellula

SUBMITTER: Raghunathan VK 

PROVIDER: S-EPMC4509060 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature