Heterozygosis deficit of polymorphic markers linked to the β-globin gene cluster region in the Iranian population.

ABSTRACT:

Objectives

Iran is considered as one of the high-prevalence areas for β-thalassemia with a rate of about 10% carrier frequency. Molecular diagnosis of the disease is performed both by direct sequencing and indirectly by the use of polymorphic markers present in the beta globin gene cluster. However, to date there is no reliable information on the application of the markers in the Iranian population. Here we report the results of an extended molecular analysis of five RFLP markers, XmnI, HindIIIA, HindIIIG, RsaI and HinfI, located within the β-globin gene cluster region in four subpopulations of Iran.

Materials and methods

A total of 552 blood samples taken from the Iranian subpopulations including Isfahan, Chaharmahal-O-Bakhtiari, Khuzestan and Hormozgan were genotyped using

SUBMITTER: Moradi T 

PROVIDER: S-EPMC4509952 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature