Metabolism of apolipoprotein A-II containing triglyceride rich ApoB lipoproteins in humans.

ABSTRACT:

Objective

To characterize human triglyceride-rich lipoproteins (TRL) with and without apoA-II and to study their metabolism in vivo.

Methods

Plasma from 11 participants on a controlled diet given a bolus infusion of [D5]l-phenylalanine to label apoB was combined into four pools and applied to anti-apoA-II immunoaffinity columns. Fractions with and without apoA-II were separated into VLDL and IDL by ultracentrifugation; lipids and apolipoproteins were measured. For kinetic measurements, apoB was isolated and hydrolyzed to the constituent amino acids. Tracer enrichment was measured by GCMS. Metabolic rates were determined by SAAM-II.

Results

VLDL and IDL with apoA-II comprised 7% and 9% of total VLDL and IDL apoB respectively. VLDL with apoA-II was enriched in apoC-III,

SUBMITTER: Desai NK 

PROVIDER: S-EPMC4509984 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature