Dynamic changes in replication timing and gene expression during lineage specification of human pluripotent stem cells.

ABSTRACT: Duplication of the genome in mammalian cells occurs in a defined temporal order referred to as its replication-timing (RT) program. RT changes dynamically during development, regulated in units of 400-800 kb referred to as replication domains (RDs). Changes in RT are generally coordinated with transcriptional competence and changes in subnuclear position. We generated genome-wide RT profiles for 26 distinct human cell types, including embryonic stem cell (hESC)-derived, primary cells and established cell lines representing intermediate stages of endoderm, mesoderm, ectoderm, and neural crest (NC) development. We identified clusters of RDs that replicate at unique times in each stage (RT signatures) and confirmed global consolidation of the genome into larger synchronously replicating segme

SUBMITTER: Rivera-Mulia JC 

PROVIDER: S-EPMC4509994 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature