Topologically associating domains and their long-range contacts are established during early G1 coincident with the establishment of the replication-timing program.

ABSTRACT: Mammalian genomes are partitioned into domains that replicate in a defined temporal order. These domains can replicate at similar times in all cell types (constitutive) or at cell type-specific times (developmental). Genome-wide chromatin conformation capture (Hi-C) has revealed sub-megabase topologically associating domains (TADs), which are the structural counterparts of replication domains. Hi-C also segregates inter-TAD contacts into defined 3D spatial compartments that align precisely to genome-wide replication timing profiles. Determinants of the replication-timing program are re-established during early G1 phase of each cell cycle and lost in G2 phase, but it is not known when TAD structure and inter-TAD contacts are re-established after their elimination during mitosis. Here, we us

SUBMITTER: Dileep V 

PROVIDER: S-EPMC4509995 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature