Isoflurane Ameliorates Acute Lung Injury by Preserving Epithelial Tight Junction Integrity.

ABSTRACT:

Background

Isoflurane may be protective in preclinical models of lung injury, but its use in patients with lung injury remains controversial and the mechanism of its protective effects remains unclear. The authors hypothesized that this protection is mediated at the level of alveolar tight junctions and investigated the possibility in a two-hit model of lung injury that mirrors human acute respiratory distress syndrome.

Methods

Wild-type mice were treated with isoflurane 1 h after exposure to nebulized endotoxin (n = 8) or saline control (n = 9) and then allowed to recover for 24 h before mechanical ventilation (MV; tidal volume, 15 ml/kg, 2 h) producing ventilator-induced lung injury. Mouse lung epithelial cells were similarly treated with isoflurane 1 h after exposure to l

SUBMITTER: Englert JA 

PROVIDER: S-EPMC4510009 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature